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Cardiovascular outcomes with SGLT-2 inhibitors and GLP-1 receptor agonist in Asians with type 2 diabetes: a systematic review and meta-analysis of cardiovascular outcome trials

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A melting curve analysis was performed from 60 °C to 85 °C, and products were run on 2% agar gels to test product specificity using GelRed loading buffer and a pre-stained DNA marker (GenScript, Piscataway, NJ)