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Improper dose titration of the particular single-action or dual-hormone GLP-1 can limit effectiveness, as the dosing is not reaching full maintenance
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[13] For the liver side specifically, researchers use hepatocyte lipid-loading models (exposing primary human hepatocytes or HepG2 cells to fatty acids such as oleate and palmitate to induce steatosis in a dish), hepatic stellate-cell lines (for example LX-2) to study fibrogenic activation, and increasingly three-dimensional systems — liver organoids, spheroids, and liver-on-a-chip devices — that better reproduce cell–cell interactions
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![Structure-Based Discovery of Orthosteric Non-Peptide GLP-1R Agonists via Integrated Virtual Screening and Molecular Dynamics[v1]](https://www.preprints.org/frontend/picture/ms_xml/manuscript/f1d5822812f016a70b1e60a239bf9397/preprints-160788-g001.png)
