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Briefly, Tni insect cells were grown in ESF 921 serum-free media to a density of 3.5 million/mL and infected with GLP1R, DNGαs and Gβ1-γ2 baculovirus at multiplicity of infection (MOI) ratio of 3:3:1
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Food or liquid getting into the lungs during surgery or other procedures that use anesthesia or deep sleepiness (deep sedation)
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