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Brain sample collection and sectioning Mice were euthanized with isoflurane (5%, Panion & BF Biotech), and subsequently perfused transcardially with PBS and paraformaldehyde (4% w/v, Electron Microscopy Sciences) for brain collection
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By stimulating both GLP-1 and GIP receptors, Tirzepatide increases insulin response when needed and reduces appetite signals more effectively than single-receptor drugs
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