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The complexes were formed by adding 10 μM peptide 15 or MEDI0382 or SAR425899 (GL Biochem), 10 μg/mL Nb35 (preparation method shown in SI Appendix , Supplementary Materials and Methods), and 25 mU/mL apyrase (New England Biolabs), incubating for 1 h at room temperature (RT)
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